Prevention of encephalomyocarditis virus-induced diabetes by live recombinant Mycobacterium bovis bacillus Calmette-Guerin in susceptible mice

Cited 11 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorBeom Kyu Choi-
dc.contributor.authorSang Hyun Cho-
dc.contributor.authorGill Han Bai-
dc.contributor.authorSang Jae Kim-
dc.contributor.authorByung Hwa Hyun-
dc.contributor.authorYong Kyung Choe-
dc.contributor.authorYong Soo Bae-
dc.date.accessioned2017-04-19T08:57:32Z-
dc.date.available2017-04-19T08:57:32Z-
dc.date.issued2000-
dc.identifier.issn0012-1797-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5288-
dc.description.abstractThe D variant of encephalomyocarditis (EMC-D) virus causes diabetes in susceptible mice by direct cytolysis of pancreatic β-cells. cDNA covering the major outer capsid protein (VP1) of the EMC-D virus was cloned into Mycobacterium bovis bacillus Calmette-Guerin (BCG). None of the SJL/J mice immunized with live recombinant BCG-VP1 (rBCG-VP1) became diabetic when challenged with the highly diabetogenic EMC-D virus, but the control mice inoculated with normal BCG developed diabetes during the same challenge. VP1-specific antibodies (including neutralizing antibodies) were markedly increased over time and reached the maximum titer at week 10 after a single immunization. The plateau of the titer lasted longer than 4 weeks. Mice and guinea pigs immunized with live rBCG-VP1 showed strong delayed-type hypersensitivity to the VP1 of the EMC-D virus. The preventive immunity still worked effectively 10 months after the primary immunization. At that time, the VP1-specific antibody was almost undetectable in the bloodstream, but a large number of VP1-specific lymphocytes was found in the spleen of the immunized mice. Our results show that live rBCG-VP1 elicits effective humoral and long-lasting cellular immune responses against EMC-D virus infection that results in the prevention of virus-induced diabetes in susceptible mice.-
dc.publisherAmer Diabetes Assoc-
dc.titlePrevention of encephalomyocarditis virus-induced diabetes by live recombinant Mycobacterium bovis bacillus Calmette-Guerin in susceptible mice-
dc.title.alternativePrevention of encephalomyocarditis virus-induced diabetes by live recombinant Mycobacterium bovis bacillus Calmette-Guerin in susceptible mice-
dc.typeArticle-
dc.citation.titleDiabetes-
dc.citation.number9-
dc.citation.endPage1467-
dc.citation.startPage1459-
dc.citation.volume49-
dc.contributor.affiliatedAuthorByung Hwa Hyun-
dc.contributor.affiliatedAuthorYong Kyung Choe-
dc.contributor.alternativeName최범규-
dc.contributor.alternativeName조상현-
dc.contributor.alternativeName배길한-
dc.contributor.alternativeName김상재-
dc.contributor.alternativeName현병화-
dc.contributor.alternativeName최용경-
dc.contributor.alternativeName배용수-
dc.identifier.bibliographicCitationDiabetes, vol. 49, no. 9, pp. 1459-1467-
dc.identifier.doi10.2337/diabetes.49.9.1459-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.