Surface-displayed viral antigens on Salmonella carrier vaccine

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Title
Surface-displayed viral antigens on Salmonella carrier vaccine
Author(s)
Jong Soo Lee; Kwang Soon Shin; Jae Gu Pan; Chul Joong Kim
Bibliographic Citation
Nature Biotechnology, vol. 18, no. 6, pp. 645-648
Publication Year
2000
Abstract
We have developed a recombinant live oral vaccine using the ice- nucleation protein (Inp) from Pseudomonas syringae to display viral antigens on the surface of Salmonella spp. Fusion proteins containing viral antigens were expressed in the oral vaccine strain, Salmonella typhi Ty21a. Surface localization was verified by immunoblotting and fluorescence-activated cell sorting. The immunogenicity of surface-displayed viral antigens on the recombinant live vaccine strain was assessed in mice inoculated intranasally and intraperitoneally. Inoculation resulted in significantly higher serum antibody level than those induced by viral antigens expressed intracellularly. Thus, this multivalent mucosal live vaccine may provide an effective means for inducing mucosal or systemic immune responses against multiple viral antigens.
Keyword
Multivalent mucosal vaccineSalmonellaSurface display
ISSN
0733-222X
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/76494
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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