Optimization of feed rate profile for the monoclonal antibody production

Abstract

The optimal control algorithm to calculate the optimal feed rate profile of nutrient solution containing two limiting nutrients was proposed. Different from other conventional optimization methods, the proposed algorithm calculated the optimal control profiles for different initial and feed conditions. The singular optimal control algorithm, dynamic programming, and nonsingular transformation algorithm were used for the optimization of simple problems of the 4th order and the performances were compared. With the proposed transformation algorithm, the final MAb concentration increased and the CPU time decreased. For the different initial glucose and glutamine conditions, the optimal control profiles were calculated with the proposed transformation algorithm. As the initial glutamine concentration increased, the final MAb concentration also increased due to the cell viability increase. This was also applied to the different feed compositions. When the glutamine concentration was increased in the feed stream, the final MAb concentration also increased.