synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus

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Title
synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus
Author(s)
Jeong Keun Kim; Heui Il Kang; Jeong Seok Chae; Young Hoon Park; Yong Jin Choi
Bibliographic Citation
FEMS Microbiology Letters, vol. 182, no. 2, pp. 313-317
Publication Year
2000
Abstract
In vitro synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus was investigated using α-ketoglutarate, L-ascorbic acid, FeSO4, S-adenosyl-L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with Proteus vulgaris GN 76/C-1 and by high performance liquid chromatography. Although the conversion rate of 7α-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox.
Keyword
7-Methoxylationcefminoxcell-free extractcephamycinproteus vulgaris GN 76/C-1streptomyces clavuligerus
ISSN
0378-1097
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1111/j.1574-6968.2000.tb08914.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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