Genomic organization of Penicillium chrysogenum chs4, a class III chitin synthase gene

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dc.contributor.authorYoon Dong Park-
dc.contributor.authorMyung Sook Lee-
dc.contributor.authorJi Hoon Kim-
dc.contributor.authorJun Namgung-
dc.contributor.authorBeom Chan Park-
dc.contributor.authorKyung Sook Bae-
dc.contributor.authorHee Moon Park-
dc.date.accessioned2017-04-19T08:57:32Z-
dc.date.available2017-04-19T08:57:32Z-
dc.date.issued2000-
dc.identifier.issn1225-8873-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5295-
dc.description.abstractClass III chitin synthases in filamentous fungi are important for hyphal growth and differentiation of several filamentous fungi. A genomic clone containing the full gene encoding Chs4, a class III chitin synthase in Penicillium chrysogenum, was cloned by PCR screening and colony hybridization from the genomic library. Nucleotide sequence analysis and transcript mapping of chs4 revealed an open reading frame (ORF) that consisted of 5 exons and 4 introns and encoded a putative protein of 915 amino acids. Nucleotide sequence analysis of the 5' flanking region of the ORF revealed a potential TATA box and several binding sites for transcription activators. The putative transcription initiation site at -716 position was identified by primer extension and the expression of the chs4 during the vegetative growth was confirmed by Northern blot analysis. Amino acid sequence analysis of the Chs4 revealed at least 5 transmembrane helices and several sites for post-translational modifications. Comparison of the amino acid sequence of Chs4 with those of other fungi showed a close relationship between P. chrysogenum and genus Aspergillus.-
dc.publisherMicrobiological Society Korea-
dc.titleGenomic organization of Penicillium chrysogenum chs4, a class III chitin synthase gene-
dc.title.alternativeGenomic organization of Penicillium chrysogenum chs4, a class III chitin synthase gene-
dc.typeArticle-
dc.citation.titleJournal of Microbiology-
dc.citation.number4-
dc.citation.endPage238-
dc.citation.startPage230-
dc.citation.volume38-
dc.contributor.affiliatedAuthorYoon Dong Park-
dc.contributor.affiliatedAuthorMyung Sook Lee-
dc.contributor.affiliatedAuthorKyung Sook Bae-
dc.contributor.alternativeName박윤동-
dc.contributor.alternativeName이명숙-
dc.contributor.alternativeName김지훈-
dc.contributor.alternativeName남궁준-
dc.contributor.alternativeName박범찬-
dc.contributor.alternativeName배경숙-
dc.contributor.alternativeName박희문-
dc.identifier.bibliographicCitationJournal of Microbiology, vol. 38, no. 4, pp. 230-238-
dc.subject.keywordChitin synthase gene-
dc.subject.keywordGenomic structure-
dc.subject.keywordPenicillium chrysogenum-
dc.subject.keywordScreening-
dc.subject.keywordTranscript mapping-
dc.subject.localChitin synthase genes-
dc.subject.localchitin synthase gene-
dc.subject.localChitin synthase gene-
dc.subject.localGenomic structure-
dc.subject.localPenicillium chrysogenum-
dc.subject.localScreening-
dc.subject.localscreening-
dc.subject.localTranscript mapping-
dc.description.journalClassY-
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