Developmental potential and transgene expression of porcine nuclear transfer embryos using somatic cells

Cited 0 time in scopus
Metadata Downloads
Title
Developmental potential and transgene expression of porcine nuclear transfer embryos using somatic cells
Author(s)
Deog Bon Koo; Yong Kook Kang; Young Hee Choi; Jung Sun Park; Ha Na Kim; Teoan Kim; Kyung Kwang Lee; Yong Mahn Han
Bibliographic Citation
Molecular Reproduction and Development, vol. 58, no. 1, pp. 15-21
Publication Year
2001
Abstract
We examined whether porcine nuclear transfer (NT) embryos carrying somatic cells have a developmental potential and NT embryos carrying transformed fibroblasts express transgenes in the preimplantation stages. In Experiment 1, different activation methods were applied to NT embryos and the development rates were examined. Relative to A23187 only or A23187/6-DMAP, electrical pulse made a significant increase in both cleavage rate (58.1 ± 13.9 or 60.7 ± 6.3 vs. 74.9 ± 7.5%) and development rate of NT embryos to the blastocyst stage (2.2 ± 2.8 or 2.2 ± 1.5 vs. 11.0 ± 4.1%). In Experiment 2, in vitro developmental competence of NT embryos was investigated. The developmental rate to the blastocyst stage of NT embryos (9.9 ± 2.4% for cumulus cells and 9.8 ± 1.6% for fibroblast cells) was significantly lower than that (22.9 ± 3.5%) of IVF-derived embryos (P < O.01). NT blastocysts derived from either cumulus (28.9 ± 11.4, n = 26) or fibroblast cells (30.2 ± 9.9, n = 27) showed smaller mean nuclei numbers than IVF-derived blastocysts (38.6 ± 10.4, n = 62) (P < 0.05). In Experiment 3, nuclear transfer of porcine fibroblasts expressing the GFP (green fluorescent protein) gene resulted in green blastocysts without losing developmental potential. These results suggest that porcine embryos reconstructed by somatic cell nuclear transfer are capable of developing to preimplantation stage. We conclude that somatic cells expressing exogenous genes can be used as nuclei donors in the production of NT-mediated transgenic pig.
Keyword
somatic cellsdevelopmental potentialnuclear transferGFPporcine
ISSN
1040-452X
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/1098-2795(200101)58:1<15::AID-MRD3>3.0.CO;2-Y
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.