Construction of artificial epithelial tissues prepared from human normal fibroblasts and C9 cervical epithelial cancer cells carrying human papillomavirus type 18 genes

Cited 3 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorEun Kyung Yang-
dc.contributor.authorSeung Won Jin-
dc.contributor.authorByung Tae Kang-
dc.contributor.authorIk Hwan Kim-
dc.contributor.authorJung Keug Park-
dc.contributor.authorSang Sook Lee-
dc.contributor.authorJin Woo Kim-
dc.contributor.authorSue Nie Park-
dc.date.accessioned2017-04-19T08:57:35Z-
dc.date.available2017-04-19T08:57:35Z-
dc.date.issued1998-
dc.identifier.issn12268372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5306-
dc.description.abstractOne cervical cancer cell line, C9, carrying human papillomavirus type 18 (HPV18) genes that is one of the major etiologic oncovirases for cervical cancer was characterized. This cell line was further characterized for its capacity related to the epithelial cell proliferation, stratification and differentiation in reconstituted artificial epithelial tissue. The in vitro construction of three dimensional artificial cervical epithelial tissue has been engineered using C9 epithelial cancer cells, human foreskin fibroblasts and a matrix made of type I collagen by organotypic culture of epithelial cells. The morphology of paraffin embedded artificial tissue was examined by histochemical staining. The artificial epithelial tissues were well developed having multilayer. However, the tissue morphology was similar to the cervical tissue having displasia induced by HPV infection. The characteristics of the artificial tissues were examined by determining the expression of specific marker proteins. In the C9 derived artificial tissues, the expression of EGF receptor, an epithelial proliferation marker proteins for stratum basale was observed up to the stratum spinosum. Another epithelial proliferation marker for stratum spinosum, cytokeratins 5/6/18, were observed well over the stratum spinosum. For the differentiation markers, the expression of involucrin and filaggrin were observed while the terminal differentiation marker, cytokeratins 10/13 were not detected at all. Therefore the reconstituted artificial epithelial tissues expressed the same types of differentiation marker proteins that are expressed in normal human cervical epithelial tissues but lacked the final differentiation capacity representing characteristics of C9 cell line as a cancer tissue derived cell line. Expression of HPV18 E6 oncoprotein was also observed in this artificial cervical epithelial tissue though the intensity of the staining was weak. Thus this artificial epithelial tissue could be used as a useful model system to examine the relationship between HPV-induced cervical oncogenesis and epithelial cell differentiation.-
dc.publisherSouth Korea-
dc.titleConstruction of artificial epithelial tissues prepared from human normal fibroblasts and C9 cervical epithelial cancer cells carrying human papillomavirus type 18 genes-
dc.title.alternativeConstruction of artificial epithelial tissues prepared from human normal fibroblasts and C9 cervical epithelial cancer cells carrying human papillomavirus type 18 genes-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number0-
dc.citation.endPage5-
dc.citation.startPage1-
dc.citation.volume3-
dc.contributor.alternativeName양은경-
dc.contributor.alternativeName진승원-
dc.contributor.alternativeName강병태-
dc.contributor.alternativeName김익환-
dc.contributor.alternativeName박정극-
dc.contributor.alternativeName이상숙-
dc.contributor.alternativeName김진우-
dc.contributor.alternativeName박순희-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 3, pp. 1-5-
dc.identifier.doi10.1007/BF02932474-
dc.subject.keywordartificial epithelial tissue-
dc.subject.keyworddifferentiation-
dc.subject.keywordhuman papillomavirus (HPV) oncogenesis-
dc.subject.keywordtissue engineering-
dc.subject.localartificial epithelial tissue-
dc.subject.localdifferentiation-
dc.subject.localDifferentiation-
dc.subject.localhuman papillomavirus (HPV) oncogenesis-
dc.subject.localtissue engineering-
dc.subject.localTissue engineering-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.