A novel epigenetic control operating on Vme1 locus leads to variegated monoallelic expression

Cited 4 time in scopus
Metadata Downloads
A novel epigenetic control operating on Vme1 locus leads to variegated monoallelic expression
Sang Kyun Jeong; Young Jae Lee; Jae Seon Jang; Chang Won Park; Jae Hoon Chung; Je Kyung Seong; Kyung Kwang Lee; Dae Yeul Yu
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 279, no. 3, pp. 884-890
Publication Year
Vme1, located near an imprinted region containing Peg1/Mest, Copg2, and Mit1/Lb9 on mouse chromosome 6, was identified and characterized to be under novel epigenetic regulations mediating nonimprinted monoallelic expression. The gene was transcribed independently from at least four promoters and alternatively spliced. Variable expression of the gene was found among individuals and was not affected by genetic backgrounds, in contrast to a relatively consistent expression of unlinked Peg3 under different genetic backgrounds. Monoallelic expression of the gene was confirmed in several tissues of hybrid F1s between a domesticus and a molossinus subspecies. The nature of monoallelic expression was different from those of its neighboring genes with respect to the allelic preference for the expression. The observed variable expression and monoallelic expression propose a mechanism that operates to variegate the Vine1 transcription acting asynchronously on parental alleles. In addition, we observed that some biallelically expressed tissues exhibited allele-specific splicing such that expression from one parental allele yields elongated splice variants, whereas the other allele is spliced into a short version. This unusual finding suggests that an epigenotype of the promoter can determine the splicing fate of the transcript.
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.