Antimicrobial Peptide of Korean Native Goat Lactoferrin and Identification of the Part Essential for This Activity

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Title
Antimicrobial Peptide of Korean Native Goat Lactoferrin and Identification of the Part Essential for This Activity
Author(s)
Michico Kimura; Myoung Soo Nam; Yoshifumi Ohkouchi; Haruto Kumura; Kei-ichi Shimazaki; Dae Yeul Yu
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 268, no. 2, pp. 333-336
Publication Year
2000
Abstract
The antimicrobial activity of lactoferrin isolated from Korean native goat (KN goat) milk was studied and its antimicrobial domain was identified using synthetic peptides. Antimicrobial activity was assayed by a micro-method using 96-well microplates and a microplate reader. The amino acid sequence of the antimicrobial domain was suggested to be YQWQRRMRKLGAPSIT and this sequence corresponds to amino acid residues 20 to 35 of KN goat lactoferrin. Five peptides with certain amino acid residues deleted were synthesized in an effort to identify the residues essential for antimicrobial activity and it was found that the part with the sequence RRMRK (24-28) is the region most important for this activity. On the other hand, the conformation of the peptides did not influence the antimicrobial activity.
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1006/bbrc.2000.2141
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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