Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences

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Title
Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences
Author(s)
Jong Sik Chun; Kyung Sook Bae
Bibliographic Citation
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, vol. 78, no. 2, pp. 123-127
Publication Year
2000
Abstract
Partial gyrA sequences were determined for twelve strains belonging to Bacillus amyloliquefaciens, B. atrophaeus, B. licheniformis, B. mojavensis, B. subtilis subsp. subtilis, B. subtilis subsp. spizizenii and B. vallismortis. The average nucleotide and translated amino acid similarities for the seven type strains were 83.7 and 95.1%, respectively, whereas the corresponding value for the 16S rRNA sequences was 99.1%. All of the type strains were sharply separated; the closest relationship was found between B. atrophaeus and B. mojavensis which shared a nucleotide similarity of 95.8%. Phylogenetic trees were inferred from gyrA nucleotide sequences using the neighbor-joining, Fitch-Margoliash and maximum parsimony algorithms. The test strains were divided into four groups, which generally reflected results previously reported in restriction digest and DNA-DNA hybridization studies. It is concluded from the comparative sequence analysis that the gyrA sequences provide a firm framework for the rapid and accurate classification and identification of Bacillus subtilis and related taxa.
Keyword
bacillus subtilisGyrAphylogenytaxonomy
ISSN
0003-6072
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1026555830014
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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