Crystallization and preliminary X-ray crystallographic analysis of NAD(+)-dependent DNA ligase from Thermus filiformis

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Title
Crystallization and preliminary X-ray crystallographic analysis of NAD(+)-dependent DNA ligase from Thermus filiformis
Author(s)
Jae Young Lee; Hyun Kyu Kim; Chang Soo Chang; Soo Hyun Eom; Kwang Yeon Hwang; Yun Je Cho; Yeon Gyu Yu; Seong Eon Ryu; Suk Tae Kwon; Se Won Suh
Bibliographic Citation
Acta Crystallographica Section D-Biological Crystallography, vol. 56, no. 3, pp. 357-358
Publication Year
2000
Abstract
A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P21, with unit-cell parameters a = 90.63, b = 117.80, c = 98.65 ?, β = 115.56°. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (V(m)) of 3.1 ?3Da-1 and a solvent content of 61%. A native data set extending to 3.0 ? resolution has been collected at 100 K using synchrotron X-rays.
ISSN
0907-4449
Publisher
Int Union Crystallography
DOI
http://dx.doi.org/10.1107/S090744490000010X
Type
Article
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1. Journal Articles > Journal Articles
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