Residual phosphate concentration under nitrogen-limiting conditions regulates curdlan production in Agrobacterium species

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dc.contributor.authorMi Kyung Kim-
dc.contributor.authorIn Young Lee-
dc.contributor.authorJung Heon Lee-
dc.contributor.authorK T Kim-
dc.contributor.authorY H Rhee-
dc.contributor.authorYoung Hoon Park-
dc.date.accessioned2017-04-19T08:57:41Z-
dc.date.available2017-04-19T08:57:41Z-
dc.date.issued2000-
dc.identifier.issn0169-4146-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5347-
dc.description.abstractWe investigated the influence of inorganic phosphate concentration on the production of curdlan by Agrobacterium species. A two-step culture method was employed where cells were first cultured, followed by curdlan production under nitrogen-limiting conditions. In the curdlan production step, cells did not grow but metabolized sugar into curdlan. Shake-flask experiments showed that the optimal phosphate concentration for curdlan production was in the range of 0.1-0.3 g I-1. As the cell concentration increased from 0.42 to 1.68 g I-1 in shake-flask cultures, curdlan production increased from 0.44 to 2.80 g I-1. However, the optimal phosphate concentration range was not0 dependent upon cell concentration. The specific production rate was about 70 mg curdlan g-cell-1 h-1 irrespective of cell concentration. When the phosphate concentration was maintained at 0.5 g I-1 under nitrogen-limiting conditions, as high as 65 g I-1 of curdlan was obtained in 120 h.-
dc.publisherSpringer-
dc.titleResidual phosphate concentration under nitrogen-limiting conditions regulates curdlan production in Agrobacterium species-
dc.title.alternativeResidual phosphate concentration under nitrogen-limiting conditions regulates curdlan production in Agrobacterium species-
dc.typeArticle-
dc.citation.titleJournal of Industrial Microbiology & Biotechnology-
dc.citation.number4-
dc.citation.endPage183-
dc.citation.startPage180-
dc.citation.volume25-
dc.contributor.affiliatedAuthorMi Kyung Kim-
dc.contributor.affiliatedAuthorIn Young Lee-
dc.contributor.affiliatedAuthorJung Heon Lee-
dc.contributor.affiliatedAuthorK T Kim-
dc.contributor.affiliatedAuthorY H Rhee-
dc.contributor.affiliatedAuthorYoung Hoon Park-
dc.contributor.alternativeName김미경-
dc.contributor.alternativeName이인영-
dc.contributor.alternativeName이중헌-
dc.contributor.alternativeName김경태-
dc.contributor.alternativeName이은혜-
dc.contributor.alternativeName박영훈-
dc.identifier.bibliographicCitationJournal of Industrial Microbiology & Biotechnology, vol. 25, no. 4, pp. 180-183-
dc.identifier.doi10.1038/sj.jim.7000053-
dc.subject.keywordagrobacterium sp-
dc.subject.keywordcurdlan-
dc.subject.keywordphosphate concentration-
dc.subject.keywordproduction-
dc.subject.localagrobacterium sp-
dc.subject.localAgrobacterium sp-
dc.subject.localAgrobacterium sp.-
dc.subject.localcurdlan-
dc.subject.localCurdlan-
dc.subject.localphosphate concentration-
dc.subject.localproduction-
dc.subject.localProduction-
dc.description.journalClassY-
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