Activation of mitogen-activated protein kinase pathways by angelan in murine macrophages

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dc.contributor.authorYoung Jin Jeon-
dc.contributor.authorSang Bae Han-
dc.contributor.authorSang Han Lee-
dc.contributor.authorHyoung Chin Kim-
dc.contributor.authorKyung Seop Ahn-
dc.contributor.authorHwan Mook Kim-
dc.date.accessioned2017-04-19T08:57:42Z-
dc.date.available2017-04-19T08:57:42Z-
dc.date.issued2001-
dc.identifier.issn15675769-
dc.identifier.uri10.1016/S1567-5769(00)00003-5ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5351-
dc.description.abstractIn our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, specifically activated macrophages to induce cytokines including inducible nitric oxide synthase (iNOS) which has strong anti-tumor activities [Immunopharmacology, 1999; 43: 1.]. In the present study, we investigated the intracellular signal transduction pathways involved in the angelan-induced iNOS synthesis by murine macrophages. Protein tyrosine phosphorylation was induced within 5 min by angelan, and the blocking of protein tyrosine kinases (PTKs) inhibited down-stream pathways leading to iNOS production in response to angelan. Treament of RAW 264.7 cells with angelan resulted in significant activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) and p38, while stress-activated protein kinase/c-Jun NH2 terminal kinase (SAPK/JNK) was not activated by angelan. The specific p38 inhibitor SB203580 abrogated the angelan-induced iNOS synthesis, whereas the selective mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase 1 (MEK-1) inhibitor PD98059 did not affect the iNOS induction. In conclusion, we demonstrate that PTK and p38 MAPK activation are required to transduce signals leading to iNOS expression in angelan-stimulated murine macrophages.-
dc.publisherElsevier-
dc.titleActivation of mitogen-activated protein kinase pathways by angelan in murine macrophages-
dc.title.alternativeActivation of mitogen-activated protein kinase pathways by angelan in murine macrophages-
dc.typeArticle-
dc.citation.titleInternational Immunopharmacology-
dc.citation.number2-
dc.citation.endPage245-
dc.citation.startPage237-
dc.citation.volume1-
dc.contributor.affiliatedAuthorHyoung Chin Kim-
dc.contributor.affiliatedAuthorKyung Seop Ahn-
dc.contributor.alternativeName전영진-
dc.contributor.alternativeName한상배-
dc.contributor.alternativeName이상한-
dc.contributor.alternativeName김형진-
dc.contributor.alternativeName안경섭-
dc.contributor.alternativeName김환묵-
dc.identifier.bibliographicCitationInternational Immunopharmacology, vol. 1, no. 2, pp. 237-245-
dc.identifier.doi10.1016/S1567-5769(00)00003-5-
dc.subject.keywordangelica gigas Nakai-
dc.subject.keywordangelan-
dc.subject.keywordmacrophages-
dc.subject.keywordp38-
dc.subject.keywordPTK-
dc.subject.localangelica gigas Nakai-
dc.subject.localAngelica gigas Nakai-
dc.subject.localangelan-
dc.subject.localAngelan-
dc.subject.localmacrophages-
dc.subject.localmacrophage-
dc.subject.localp38-
dc.subject.localPTK-
dc.description.journalClassY-
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > 1. Journal Articles
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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