Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze Hye

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Title
Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze Hye
Author(s)
Hye Kyong Kim; Sei Ryang Oh; Hyeong Kyu Lee; Hoon Huh
Bibliographic Citation
Biotechnology Letters, vol. 23, no. 1, pp. 55-60
Publication Year
2001
Abstract
Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g-1 dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10000) at 50 μg ml-1 elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.
Keyword
agastache rugosabenzothiadiazolerosmarinic acidsuspension cultureyeast elicitor
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1026738409671
Type
Article
Appears in Collections:
Ochang Branch Institute > 1. Journal Articles
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