A thermostable xylose isomerase from Thermus thermophilus: biochemical characterization, crystallization, and preliminary X-ray analyses

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dc.contributor.authorChang Soo Chang-
dc.contributor.authorByoung Chul Park-
dc.contributor.authorDae Sil Lee-
dc.contributor.authorSe Won Suh-
dc.date.accessioned2017-04-19T08:57:44Z-
dc.date.available2017-04-19T08:57:44Z-
dc.date.issued1998-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5369-
dc.description.abstractA highly thermostable xylose isomerase from Thermus thermophilus has been expressed in Escherichia coli and crystallized. The purified enzyme shows its optimum temperature at 90°C. It has been crystallized at room temperature using polyethylene glycol 4000 as the precipitant. The crystal belongs to the orthorhombic space group P212121, with unit cell parameters of a = 73.34 ?, b = 144.05 ?, c = 155.07 ?. The presence of one molecule of tetrameric xylose isomerase in the asymmetric unit gives a crystal volume per protein mass (Vm) of 2.32 ?3/Da and the solvent content of 47.0% by volume. The diffraction pattern extends to 1.9 ? Bragg spacing with synchrotron radiation and a set of native data has been collected to 2.3 ?.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleA thermostable xylose isomerase from Thermus thermophilus: biochemical characterization, crystallization, and preliminary X-ray analyses-
dc.title.alternativeA thermostable xylose isomerase from Thermus thermophilus: biochemical characterization, crystallization, and preliminary X-ray analyses-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number6-
dc.citation.endPage603-
dc.citation.startPage600-
dc.citation.volume31-
dc.contributor.affiliatedAuthorByoung Chul Park-
dc.contributor.affiliatedAuthorDae Sil Lee-
dc.contributor.alternativeName장창수-
dc.contributor.alternativeName박병철-
dc.contributor.alternativeName이대실-
dc.contributor.alternativeName서세원-
dc.identifier.bibliographicCitationBMB Reports, vol. 31, no. 6, pp. 600-603-
dc.subject.keywordcrystallization-
dc.subject.keywordthermostable enzyme-
dc.subject.keywordthermus thermopilus-
dc.subject.keywordxylose isomerase-
dc.subject.localCrystallization-
dc.subject.localcrystallization-
dc.subject.localthermostable enzyme-
dc.subject.localThermostable enzyme-
dc.subject.localthermus thermopilus-
dc.subject.localXylose isomerase-
dc.subject.localxylose isomerase-
dc.description.journalClassY-
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Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
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