Nucleotide analysis of Phaffia rhodozyma DNA fragment that functions as ARS in Saccharomyces cerevisiae

Cited 0 time in scopus
Metadata Downloads
Title
Nucleotide analysis of Phaffia rhodozyma DNA fragment that functions as ARS in Saccharomyces cerevisiae
Author(s)
Hee Young Chung; Min Hee Hong; Young Hyun Chun; Suk Bai; Suhn Young Im; Hwang Hee Blaise Lee; Jong Chun Park; Dong Ho Kim; Soon Bai Chun
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 8, no. 6, pp. 650-655
Publication Year
1998
Abstract
The chromosomal DNA fragment from Phaffia rhodozyma CBS 6938 which is able to autonomously replicate in the yeast Saccharomyces cerevisiae was cloned on an integrative URA3 plasmid. Its minimal fragment exhibiting autonomously replicating activity in the S. cerevisiae gave a higher frequency transformation efficiency than that found for centromere-based plasmid, and enabled extrachromosomally stable transmission of the plasmids in one copy per yeast cell under non-selective culture condition. The 836-bp DNA element lacked an ORF and did not contain any acceptable match to an ARS core consensus. Sequence analysis, however, displayed a cluster of three hairpin-loop-sequences with individual ΔG25°C free energy value of - 10.0, -17.5, and -17.0 kcal · mol-1 as well as a 9-bp sequence with two base pair mismatches to the S. cerevisiae/E. coli gyrase-binding site. This 836-bp sequence also included one 7-bp sequence analogous to the core consensus of centromeric DNA element III (CD) of S. cerevisiae, but CD-like 7 bp sequence alone did not give a replicative function in this yeast.
Keyword
nucleotide sequence analysisphaffia rhodozymareplicative DNA fragmentsaccharomyces cerevisiae
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.