Development of a screening system for drugs against human papillomavirus-associated cervical cancer: based on E7-Rb binding

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Title
Development of a screening system for drugs against human papillomavirus-associated cervical cancer: based on E7-Rb binding
Author(s)
Young Sik Cho; Cheong Weon Cho; Jeong Woo Kang; Min Chul Cho; Kyung Ae Lee; Jung Hyun Shim; Dur Han Kwon; Yong Kyung Choe; Sue Nie Park; Do Young Yoon
Bibliographic Citation
BMB Reports, vol. 34, no. 1, pp. 80-84
Publication Year
2001
Abstract
The human papillomavirus E7 protein can form a specific complex with a retinoblastoma tumor suppressor gene product (p105-Rb) that results in the release of the E2F transcription factor, which is critical for the growth-deregulation and transforming properties of the viral E7 oncoprotein. In an attempt to apply interaction between the E7 oncoprotein and a target cellular protein Rb for an in vitro screening system for drugs against human papillomavirus infection, we primarily investigated the E7-Rb binding through a pull down assay and enzyme-linked immunosorbent assay. The pull down assay showed that both glutathione S-transferase-tagged E7 and His-tagged E7 immobilized on resins specifically produced complexes with bacterially expressed Rb in a dose-dependent manner, as determined by immunoblot analyses. This result coincided with that of an enzyme-linked immunosorbent assay, which is a useful system for the mass screening of potential drugs. Taken together, this screening system (based on the interaction between E7 and Rb) can be a promising system in the development of drugs against cervical cancers caused by human papillomavirus infection.
Keyword
E7ELISAHPVRb
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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