Molecular cloning and recombinant expression of the long form of leptin receptor(Ob-Rb) cDNA as isolated from rat spleen

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Title
Molecular cloning and recombinant expression of the long form of leptin receptor(Ob-Rb) cDNA as isolated from rat spleen
Author(s)
Sung Kyu Ju; Jung Hyun Park; Shin Young Na; Kwan Hee You; Kil Lyong Kim; Myung Kyu Lee
Bibliographic Citation
BMB Reports, vol. 34, no. 2, pp. 156-165
Publication Year
2001
Abstract
Leptin is a circulating non-glycosylated protein that is mainly produced in adipocytes. Leptin acts in the brain to regulate food intake and energy expenditure. Previously we reported our success in the isolation of a partial cDNA of the long form of the leptin receptor, OB-Rb, from rat spleen, and showed that leptin might also play a role in peripheral immune organs. In the present study, for the first time, the complete coding region of OB-Rb cDNA was cloned from rat splenocytes, and its nucleotide sequence was determined. The cDNA was then further expressed in E. coli and mammalian cells, thereby confirming the functional integrity of this receptor. Prokaryotically overexpressed OB-R protein was then used as an immunizing antigen in BALB/c mice to produce leptin receptor-specific antibodies. By using them, we confirmed the cell surface expression of OB-Rb in transfected CHO cells. It is our belief that the reagents, as produced in this study, will be of great use in further studies of the biological role of rat leptin.
Keyword
leptin receptorrecombinant expressionOB-Rbtransfection
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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