A sesquiterpene lactone, costunolide, from Magnolia grandiflora inhibits NF-κB by targeting IκB phosphorylation

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Title
A sesquiterpene lactone, costunolide, from Magnolia grandiflora inhibits NF-κB by targeting IκB phosphorylation
Author(s)
Tae Hyeon Koo; Jeong Hyung Lee; Yun Joo Park; Young Soo Hong; Hang Sub Kim; Kyu Won Kim; Jung Joon Lee
Bibliographic Citation
Planta Medica, vol. 67, no. 2, pp. 103-107
Publication Year
2001
Abstract
A sesquiterpene lactone, costunolide (CTN), was identified from Magnolia grandiflora together with parthenolide (PTN) by its strong inhibition of LPS-induced NF-κB activation. CTN, which showed more potent inhibition than PTN in the NF-κB activation, strongly suppressed nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. RT-PCR and Western blot analyses demonstrated that CTN suppressed the expression of iNOS mRNA and protein in a dose-dependent manner. CTN also significantly inhibited LPS-induced DNA-binding activity of NF-κB as well as the LPS-induced degradation of IκB-α and -β. Furthermore, CTN inhibited LPS-induced phosphorylation of IκB-α. These findings support that CTN inhibits NO production by down-regulating iNOS expression, at least, in part through the inhibition of IκBs' phosphorylation and degradation, which are essential for the activation of NF-κB.
Keyword
magnolia grandifloramagnoliaceaecostunolideinducible nitric oxide synthaseNF-κBIκB
ISSN
0032-0943
Publisher
Georg Thieme Verlag Kg
DOI
http://dx.doi.org/10.1055/s-2001-11503
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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