Kaurane diterpenes from Isodon japonicus inhibit nitric oxide and prostaglandin E2 production and NF-κB activation in LPS-stimulated macrophage RAW264.7 cells

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Title
Kaurane diterpenes from Isodon japonicus inhibit nitric oxide and prostaglandin E2 production and NF-κB activation in LPS-stimulated macrophage RAW264.7 cells
Author(s)
Bang Yeon Hwang; Jeong Hyung Lee; Tae Hyeon Koo; Hang Sub Kim; Young Soo Hong; Jai Seup Ro; Kyong Soon Lee; Jung Joon Lee
Bibliographic Citation
Planta Medica, vol. 67, no. 5, pp. 406-410
Publication Year
2001
Abstract
A methanolic extract of the whole plant of Isodon japonicus (Labiatae) showed potent inhibition on the LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production in RAW264.7 cells. Four known kaurane diterpenes were isolated by activity-guided fractionation and their structures were identified as kamebanin (1), kamebacetal A (2), kamebakaurin (3), excisanin A (4). All compounds also inhibited the LPS-induced NF-κB activation as assessed by NF-κB reporter assay and electrophoretic mobility shift assay (EMSA). Compounds 2-4 showed comparable inhibitory effects on the LPS-induced production of NO and PGE2, and activation of NF-κB without affecting cell viability. These results suggest that kaurane diterpenes could exert their inhibitory effects on the production of NO and PGE2 through the suppression of NF-κB activation, and be partially responsible for the anti-inflammatory activities of the genus Isodon.
Keyword
Isodon japonicusKaurane diterpenesLabiataeNitric oxideProstaglandin E2, NF-κB
ISSN
0032-0943
Publisher
Georg Thieme Verlag Kg
DOI
http://dx.doi.org/10.1055/s-2001-15808
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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