Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis

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Title
Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis
Author(s)
Ki Hyo Jang; Ki Bang Song; Chul Ho Kim; Bong Hyun Chung; Soon Ah Kang; Uck Han Chun; Ryo Won Choue; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 23, no. 5, pp. 339-344
Publication Year
2001
Abstract
The characteristics of levan formation by different preparations of levansucrase (free and immobilized enzyme and toluene-permeabilized whole cells), derived from recombinant levansucrase from Zymomonas mobilis expressed in Escherichia coli, were investigated. The maximal yield of levan by the three preparations were similar and were about 70-80% on a fructose-released basis with sucrose as nutrient at 100 g l-1. Immobilized enzyme and toluene-permeabilized whole cells produced low molecular weight levan (2-3 × 106), as determined by HPLC while high molecular weight levan (>6 × 106) was the major product with the free levansucrase. The size of levan can thus be controlled by immobilized levansucrase and toluene-permeabilized whole cells in high yield.
Keyword
immobilizationlevansucraselow molecular weight levanpermeabilization
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1005641220946
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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