Enhanced transformation efficiency of an anticoagulant hirudin gene into Saccharomyces cerevisiae by a double δ-sequence

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Title
Enhanced transformation efficiency of an anticoagulant hirudin gene into Saccharomyces cerevisiae by a double δ-sequence
Author(s)
Myoung Dong Kim; Young Je Yoo; Sang Ki Rhee; Jin Ho Seo
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 11, no. 1, pp. 61-64
Publication Year
2001
Abstract
Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double δ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single δ system.
Keyword
Hirudinδ-intergrationSaccharomyces cerevisiaetransformation efficiency
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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