Prediction of the secondary structure of the AgfA subunit of Salmonella enteritidis overexpressed as an MBP-fused protein

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Title
Prediction of the secondary structure of the AgfA subunit of Salmonella enteritidis overexpressed as an MBP-fused protein
Author(s)
Mi Sun Won; So Youn Kim; Seung Hwan Lee; Chul Jung Kim; Hyun Su Kim; Moo Hyung Jun; Kyung Bin Song
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 11, no. 1, pp. 164-166
Publication Year
2001
Abstract
To examine the characteristics of the recombinant thin aggregative fimbriae of Salmonella, the AgfA subunit gene was amplified from Salmonella enteritidis using a PCR. The maltose binding protein (MBP)-AgfA fusion protein was overproduced in E. coli and purified. The secondary structure of AgfA was then elucidated from the difference CD spectra. An estimation of the secondary structure of AgfA using the self-consistent method revealed a mostly β-sheet structure.
Keyword
secondary structuresalmonella enteritidisAgfA
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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