Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cereΩisiae by chromosomal δ-integration

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dc.contributor.authorMyoung Dong Kim-
dc.contributor.authorSang Ki Rhee-
dc.contributor.authorJin Ho Seo-
dc.date.accessioned2017-04-19T08:57:54Z-
dc.date.available2017-04-19T08:57:54Z-
dc.date.issued2001-
dc.identifier.issn01681656-
dc.identifier.uri10.1016/S0168-1656(00)00376-Xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5441-
dc.description.abstractRecombinant Saccharomyces cerevisiae strains were developed to overproduce an anticoagulant hirudin. The δ-sequences of the yeast retrotransposon Ty1 and URA3 were used as target sites for a hirudin expression cassette. High copy-number transformants were successfully selected using a dominant selection antibiotic, G418. The copy numbers of the hirudin expression cassette integrated into δ-sequences of the yeast chromosome ranged from five to ten copies per cell. Production of hirudin in the δ-integrated recombinant S. cerevisiae system increased over two-fold compared with the YEp-based episomal hirudin expression system. A linear relationship between the copy number of the hirudin expression cassette and hirudin expression level was observed up to 10 copies. The hirudin expression cassettes integrated into the yeast chromosome were stably maintained in non-selective culture conditions.-
dc.publisherElsevier-
dc.titleEnhanced production of anticoagulant hirudin in recombinant Saccharomyces cereΩisiae by chromosomal δ-integration-
dc.title.alternativeEnhanced production of anticoagulant hirudin in recombinant Saccharomyces cereΩisiae by chromosomal δ-integration-
dc.typeArticle-
dc.citation.titleJournal of Biotechnology-
dc.citation.number1-
dc.citation.endPage48-
dc.citation.startPage41-
dc.citation.volume85-
dc.contributor.alternativeName김명동-
dc.contributor.alternativeName이상기-
dc.contributor.alternativeName서진호-
dc.identifier.bibliographicCitationJournal of Biotechnology, vol. 85, no. 1, pp. 41-48-
dc.identifier.doi10.1016/S0168-1656(00)00376-X-
dc.subject.keywordhirudin-
dc.subject.keywordSaccharomyces cerevisiae-
dc.subject.keywordmitotic stability-
dc.subject.keywordδ-integration-
dc.subject.keywordcopy number-
dc.subject.localhirudin-
dc.subject.localHirudin-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localmitotic stability-
dc.subject.localδ-integration-
dc.subject.localcopy number-
dc.description.journalClassY-
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