Production mechanism of anti-phospholipid antibody and analysis of its recongnition profile = Anti-phospholipid antibody의 생성 기전과 인지질 인식 형태에 관한 해석

Abstract

Anti-phosohatidylserine(anti-PS) monoclonal antibodies of the IgG isotype in the serum of normal Balb/c mouse were effectively induced by the intrasplenic immunization of phosphatidylserine(PS), while only a slight increase of the titer was observed when the antigen was injected intravenously. Among the fifteen monoclonal antibodieds(mAbs) examined, one mAb designated PSG3(IgG2b, κ chain), showed a strong lupus anticoagulant, ss-DNA, ds-DNA binding and HUVEC(human umbilical vein endothelial cell) binding activities. V region of the heavy chain of PSG3 was sequenced by a modification of Sanger's dideoxynucleotide method of DNA sequencing, using 5 crude mRNA as template, synthetic oligonucleotides as primers and reverse transcriptase to incorporate both dideoxynucleotides and labeled deoxynucleotides. V region of the heavy chain of PSG3 showed high homology with J606 which recognized β(2→1) fructosan of bacteria membrane. It raises the possibility that IgG class anti-phospholipid antibody(PSG3) is generated, by gene mutation, from B cells previously activated by bacteria.