Production mechanism of anti-phospholipid antibody and analysis of its recongnition profile = Anti-phospholipid antibody의 생성 기전과 인지질 인식 형태에 관한 해석

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Title
Production mechanism of anti-phospholipid antibody and analysis of its recongnition profile = Anti-phospholipid antibody의 생성 기전과 인지질 인식 형태에 관한 해석
Author(s)
Kyung Soo Nam
Bibliographic Citation
Korean Journal of Immunology, vol. 14, no. 2, pp. 187-192
Publication Year
1992
Abstract
Anti-phosohatidylserine(anti-PS) monoclonal antibodies of the IgG isotype in the serum of normal Balb/c mouse were effectively induced by the intrasplenic immunization of phosphatidylserine(PS), while only a slight increase of the titer was observed when the antigen was injected intravenously. Among the fifteen monoclonal antibodieds(mAbs) examined, one mAb designated PSG3(IgG2b, κ chain), showed a strong lupus anticoagulant, ss-DNA, ds-DNA binding and HUVEC(human umbilical vein endothelial cell) binding activities. V region of the heavy chain of PSG3 was sequenced by a modification of Sanger's dideoxynucleotide method of DNA sequencing, using 5 crude mRNA as template, synthetic oligonucleotides as primers and reverse transcriptase to incorporate both dideoxynucleotides and labeled deoxynucleotides. V region of the heavy chain of PSG3 showed high homology with J606 which recognized β(2→1) fructosan of bacteria membrane. It raises the possibility that IgG class anti-phospholipid antibody(PSG3) is generated, by gene mutation, from B cells previously activated by bacteria.
ISSN
I000-0015
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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