High frequency somatic embryogenesis and plant regeneration in zygotic embryo cultures of Liriope platyphylla Wang et Tang

Abstract

Mature zygotic embryos of Liriope platyphylla Wang et Tang formed embryogenic calluses at a frequency of 33% when cultured on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid. Upon transfer to half-strength MS basal medium, embryogenic calluses gave rise to numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were successfully transferred to potting soil, where they were subsequently grown to maturity.