Characteristics and glycerol metabolism of fumarate-reducing Enterococcus faecalis RKY1

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Title
Characteristics and glycerol metabolism of fumarate-reducing Enterococcus faecalis RKY1
Author(s)
Hwa Won Ryh; Kui Hyun Kang; Jae Gu Pan; Ho Nam Chang
Bibliographic Citation
Biotechnology and Bioengineering, vol. 72, no. 1, pp. 119-124
Publication Year
2001
Abstract
Enterococcus faecalis RKY1, which converts fumarate to succinate with a high yield, was identified on the basis of a phylogenetic analysis of the 16S rDNA gene sequence. The strain was incubated at 38°C for 18 h to examine the possible diversion of glucose or glycerol fermentation by fumarate. The products of glucose and glycerol fermentation with fumarate were quite different from those of normal fermentation, which ultimately produces lactate, in that mainly succinate is produced. Metabolic pathway stoichiometry was used to analyze the oxidation of glycerol to succinate by Enterococcus faecalis RKY1. The stoichiometric relationship between glycerol and fumarate was used as a guideline to accumulate succinate more efficiently.
Keyword
enterococcus faecalisbioconversionsuccinatefumarateglycerol
ISSN
0006-3592
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/1097-0290(20010105)72:1<119::AID-BIT15>3.0.CO;2-0
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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