Phosphorylation and Activation of Phospholipase D1 by Protein Kinase C in Vivo: Determination of Multiple Phosphorylation Sites

Cited 69 time in scopus
Metadata Downloads
Title
Phosphorylation and Activation of Phospholipase D1 by Protein Kinase C in Vivo: Determination of Multiple Phosphorylation Sites
Author(s)
Yong Kim; Jung Min Han; Jong Bae Park; Sang Do Lee; Yong Seok Oh; Chu Ro Chung; Tae Hoon G Lee; Jae Ho Kim; Seung Kiel Park; Jong Shin Yoo; Pann Gill Suh; Sung Ho Ryu
Bibliographic Citation
Biochemistry, vol. 38, no. 32, pp. 10344-10351
Publication Year
1999
Abstract
Protein kinase C (PKC) is an important regulator of phospholipase D1 (PLD1). Currently there is some controversy about a phosphorylation-dependent or -independent mechanism of the activation of PLD1 by PKC. To solve this problem, we examined whether PLD1 is phosphorylated by PKC in vivo. For the first time, we have now identified multiple basal phophopeptides and multiple phorbol myristate acetate (PMA) induced phosphopeptides of endogenous PLD1 in 3Y1 cells as well as of transiently expressed PLD1 in COS-7 cells. Down regulation or inhibition of PKC greatly attenuated the PMA-induced phosphorylation as well as the activation of PLD1. In the presence of PMA, purified PLD1 from rat brain was also found to be phosphorylated by PKCα in vitro at multiple sites generating seven distinct tryptic phosphopeptides. Four phosphopeptides generated in vivo and in vitro correlated well with each other, suggesting direct phosphorylation of PLD1 by PKCα in the cells. Serine 2, threonine 147, and serine 561 were identified as phosphorylation sites, and by mutation of these residues to alanine these residues were proven to be specific phosphorylation sites in vivo. Interestingly, threonine 147 is located in the PX domain and serine 561 is in the negative regulatory 'loop' region of PLD1. Mutation of serine 2, threonine 147, or serine 561 significantly reduced PMA-induced PLD1 activity. These results strongly suggest that phosphorylation plays a pivotal role in PLD1 regulation in vivo.
ISSN
0006-2960
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/bi990579h
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.