Secretion and localization of invertase and inulinase in recombinant Saccharomyces-cerevisiae

Cited 29 time in scopus
Metadata Downloads
Title
Secretion and localization of invertase and inulinase in recombinant Saccharomyces-cerevisiae
Author(s)
Soo Wan Nam; Koji Yoda; Makari Yamasaki
Bibliographic Citation
Biotechnology Letters, vol. 15, no. 10, pp. 1049-1054
Publication Year
1993
Abstract
Secretion of invertase and inulinase produced by recombinant Saccharomyces cerevisiae cells were investigated under derepression conditions of GALI promoter. Secreted invertase mainly localized in the periplasmic space, but most of inulinase was found in the extracellular culture medium. This high level of extracellular secretion of inulinase was not dependent on the growth phase in which derepression of GALI promoter occurs. Our results indicate that the inulinase polypeptide itself may have a function for the protein secretion into the culture medium.
Keyword
inulinasesaccharomyces cerevisiaesecretion
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00129936
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.