Interleukin-4 signals regulating CD23 gene-expression in human B cells : protein kinase C-independent signaling pathways

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Title
Interleukin-4 signals regulating CD23 gene-expression in human B cells : protein kinase C-independent signaling pathways
Author(s)
Choong Eun Lee; Suk Ran Yoon; Kwang Ho Pyun
Bibliographic Citation
Cellular Immunology, vol. 146, no. 1, pp. 171-185
Publication Year
1993
Abstract
Signal transduction by IL-4 leading to the activation of CD23(FcεRII) gene expression using human tonsillar B cells was studied. IL-4 stimulated CD23 mRNA transcription within hours (1-4 hr) which preceded the later induction of cell surface CD23. The induction of CD23 gene transcription by IL-4 was not adversely affected by cycloheximide, suggesting that post-translational modifications are accounted for the gene activation. PKC activators (PMA, diacylglycerol, indolactam) were effective inducers of CD23 gene expression, whereas calcium ionophores were not. PMA and IL-4 also displayed similar induction kinetics for CD23 mRNA. However, the signaling pathways utilized by the two agents appear distinct as shown by (1) cotreatment of IL4 and PMA caused CD23 gene expression over the maximum level inducible by each agent alone and (2) unlike the PMA-induced CD23 expression, the IL-4-induced expression was not affected by PKC inhibitors. These results strongly suggest that IL-4 signals leading to CD23 gene activation are mediated via a PKC-independent pathway. A possible role of tyrosine kinases in the regulation of CD23 expression is discussed.
ISSN
0008-8749
Publisher
Elsevier
DOI
http://dx.doi.org/10.1006/cimm.1993.1015
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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