Lipase reaction in AOT-isooctane reversed micelles : effect of water on equilibria

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Title
Lipase reaction in AOT-isooctane reversed micelles : effect of water on equilibria
Author(s)
Dae Seok Han; Joon Shick Rhee; Sun Bok Lee
Bibliographic Citation
Biotechnology and Bioengineering, vol. 30, no. 3, pp. 381-388
Publication Year
1987
Abstract
Equations corresponding to a two-substrate, second-order reversible model are derived and used for further analysis. The progress curves predicted from the rate equations agree very well with the experimental results under various reaction conditions. The values of the molar ratio of water to surfactant (R) which maximize the initial reaction rate and maximum fractional conversion is predictable from the derived rate equations and the resulting relationship between R and the kinetic constants. The effect of water on equilibria for hydrolytic reaction in reversed micelles has been investigated using lipase as a model enzyme.
Keyword
chemical reactions - hydrolysisAOT isooctanelipasereversed micelles
ISSN
0006-3592
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/bit.260300309
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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