A novel dual-specificity protein kinase targeted to the chloroplast in tobacco

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Title
A novel dual-specificity protein kinase targeted to the chloroplast in tobacco
Author(s)
Hye Sun Cho; Gyeong Mee Yoon; Sang Sook Lee; Young A. Kim; In Hwan Hwang; Do Il Choi; Hyun Sook Pai
Bibliographic Citation
FEBS Letters, vol. 497, no. 2, pp. 124-130
Publication Year
2001
Abstract
The NtDSK1 cDNA encoding a novel chloroplast-targeted protein kinase was identified in Nicotiana tabacum. It contains the kinase domain at the C-terminus and a putative regulatory domain at the N-terminus. The recombinant NtDSK1 underwent autophosphorylation of serine, threonine, and tyrosine residues, indicating that NtDSK1 encodes a functional dual-specificity protein kinase. The NtDSK1-green fluorescent protein fusion protein was targeted to chloroplasts. Furthermore, the NtDSK1 protein was immunodetected in chloroplast fractions isolated from tobacco seedlings. The NtDSK1 mRNA expression was developmentally regulated in different tissues, including anthers and germinating seeds, and strongly stimulated by gibberellin. The mRNA was rapidly light responsive during seedling growth. NtDSK1 may play a role in a light-regulated signaling process in tobacco.
Keyword
chloroplast-targeteddual-specificity protein kinaselight- and gibberellin-stimulated expressionNtDSK1tobacco
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/S0014-5793(01)02439-5
Type
Article
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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