Hepatitis C virus core protein transactivates insulin-like growth factor II gene transcription through acting concurrently on Egr1 and Sp1 sites

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dc.contributor.authorSook Lee-
dc.contributor.authorUi Sun Park-
dc.contributor.authorYoung Ik Lee-
dc.date.accessioned2017-04-19T08:58:17Z-
dc.date.available2017-04-19T08:58:17Z-
dc.date.issued2001-
dc.identifier.issn0042-6822-
dc.identifier.uri10.1006/viro.2001.0892ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5541-
dc.description.abstractThe possibility that hepatitis C virus core gene product (HCV-core) acts as a transactivator in insulin-like growth factor II (IGF-II) gene transcription was tested. HCV-core protein increases endogenous IGF-II expression from promoter 4 (P4) of the IGF-II gene through two cis-acting elements: Sp1 and Egr1 binding sites. Sp1 and Egr1 both bind to IGF-II P4 and functionally cooperate in mediating the maximal activity of IGF-II P4. HCV-core protein induced the binding of Sp1 and Egr1 on its binding sites on IGF-II P4. In addition, Sp1 and Egr1 were stimulated to phosphorylate by HCV-core, and its DNA binding activity was up-regulated upon HCV-core transfection. Transfection with HCV-core in HepG2 cells stimulated the membrane translocation of protein kinase C (PKC) and the treatment of HCV-core transfected cells with calphostin C, a PKC inhibitor, blocked induction of Sp1 and Egr1 DNA binding activity, and eventually transcriptional transactivations of the IGF-II gene. Increasing the DNA binding activity of the phosphorylated form of Sp1 and Egr1 might be an important mechanism for regulating IGF-II gene expression and for promoting cell division during hepatic carcinogenesis. These results indicate that HCV-core functions as a positive regulator of IGF-II transcription through the PKC pathway and that Sp1 and Egr1 are direct targets of the transcriptional regulation of the IGF-II gene which plays an important role in hepatitis C virus pathogenesis during the formation of hepatocellular carcinoma (HCC).-
dc.publisherElsevier-
dc.titleHepatitis C virus core protein transactivates insulin-like growth factor II gene transcription through acting concurrently on Egr1 and Sp1 sites-
dc.title.alternativeHepatitis C virus core protein transactivates insulin-like growth factor II gene transcription through acting concurrently on Egr1 and Sp1 sites-
dc.typeArticle-
dc.citation.titleVirology-
dc.citation.number2-
dc.citation.endPage177-
dc.citation.startPage167-
dc.citation.volume238-
dc.contributor.affiliatedAuthorSook Lee-
dc.contributor.affiliatedAuthorUi Sun Park-
dc.contributor.affiliatedAuthorYoung Ik Lee-
dc.contributor.alternativeName이숙-
dc.contributor.alternativeName박의선-
dc.contributor.alternativeName이영익-
dc.identifier.bibliographicCitationVirology, vol. 238, no. 2, pp. 167-177-
dc.identifier.doi10.1006/viro.2001.0892-
dc.description.journalClassY-
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