DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sook Lee | - |
dc.contributor.author | Ui Sun Park | - |
dc.contributor.author | Young Ik Lee | - |
dc.date.accessioned | 2017-04-19T08:58:17Z | - |
dc.date.available | 2017-04-19T08:58:17Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0042-6822 | - |
dc.identifier.uri | 10.1006/viro.2001.0892 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5541 | - |
dc.description.abstract | The possibility that hepatitis C virus core gene product (HCV-core) acts as a transactivator in insulin-like growth factor II (IGF-II) gene transcription was tested. HCV-core protein increases endogenous IGF-II expression from promoter 4 (P4) of the IGF-II gene through two cis-acting elements: Sp1 and Egr1 binding sites. Sp1 and Egr1 both bind to IGF-II P4 and functionally cooperate in mediating the maximal activity of IGF-II P4. HCV-core protein induced the binding of Sp1 and Egr1 on its binding sites on IGF-II P4. In addition, Sp1 and Egr1 were stimulated to phosphorylate by HCV-core, and its DNA binding activity was up-regulated upon HCV-core transfection. Transfection with HCV-core in HepG2 cells stimulated the membrane translocation of protein kinase C (PKC) and the treatment of HCV-core transfected cells with calphostin C, a PKC inhibitor, blocked induction of Sp1 and Egr1 DNA binding activity, and eventually transcriptional transactivations of the IGF-II gene. Increasing the DNA binding activity of the phosphorylated form of Sp1 and Egr1 might be an important mechanism for regulating IGF-II gene expression and for promoting cell division during hepatic carcinogenesis. These results indicate that HCV-core functions as a positive regulator of IGF-II transcription through the PKC pathway and that Sp1 and Egr1 are direct targets of the transcriptional regulation of the IGF-II gene which plays an important role in hepatitis C virus pathogenesis during the formation of hepatocellular carcinoma (HCC). | - |
dc.publisher | Elsevier | - |
dc.title | Hepatitis C virus core protein transactivates insulin-like growth factor II gene transcription through acting concurrently on Egr1 and Sp1 sites | - |
dc.title.alternative | Hepatitis C virus core protein transactivates insulin-like growth factor II gene transcription through acting concurrently on Egr1 and Sp1 sites | - |
dc.type | Article | - |
dc.citation.title | Virology | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 177 | - |
dc.citation.startPage | 167 | - |
dc.citation.volume | 238 | - |
dc.contributor.affiliatedAuthor | Sook Lee | - |
dc.contributor.affiliatedAuthor | Ui Sun Park | - |
dc.contributor.affiliatedAuthor | Young Ik Lee | - |
dc.contributor.alternativeName | 이숙 | - |
dc.contributor.alternativeName | 박의선 | - |
dc.contributor.alternativeName | 이영익 | - |
dc.identifier.bibliographicCitation | Virology, vol. 238, no. 2, pp. 167-177 | - |
dc.identifier.doi | 10.1006/viro.2001.0892 | - |
dc.description.journalClass | Y | - |
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