Influence of oocyte nuclei on demethylation of donor genome in cloned bovine embryos

Cited 45 time in scopus
Metadata Downloads
Title
Influence of oocyte nuclei on demethylation of donor genome in cloned bovine embryos
Author(s)
Yong Kook Kang; Deog Bon Koo; Jung Sun Park; Young Hee Choi; Kyung Kwang Lee; Yong Mahn Han
Bibliographic Citation
FEBS Letters, vol. 499, no. 1, pp. 55-58
Publication Year
2001
Abstract
We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bovine embryos. Here, we examined, using bisulfite-sequencing technology, whether the inefficient demethylation of cloned donor genomes could be rescued by the presence of oocytic nuclei. Both AciI digestion and sequencing analyses showed that satellite sequence was demethylated more efficiently in cloned tetraploid blastocysts than in diploid clones. When methyl-CpG density (the number of methyl-CpG sites per string) was scored, a significant decrease was observed in tetraploids (P < 0.001). These results suggest that unknown mechanisms provided by oocytic nuclei could assist the demethylation of satellite sequences in tetraploid clones.
Keyword
epigeneticnuclear transferbisulfitereprogramming
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/S0014-5793(01)02514-5
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.