Both e6 and e7 oncoproteins of human papillomavirus 16 inhibit il-18-induced IFN-γ production in human peripheral blood mononuclear and NK cells

Cited 91 time in scopus
Metadata Downloads
Title
Both e6 and e7 oncoproteins of human papillomavirus 16 inhibit il-18-induced IFN-γ production in human peripheral blood mononuclear and NK cells
Author(s)
Shin Je Lee; Young Sik Cho; Min Chul Cho; Jung Hyun Shim; Kyung Ae Lee; Kwang Kjune Ko; Yong Kyung Choe; Sue Nie Park; Tomoaki Hoshino; Soo Hyun Kim; Charles A Dinarello; Do Young Yoon
Bibliographic Citation
Journal of Immunology, vol. 167, no. 1, pp. 497-504
Publication Year
2001
Abstract
Cervical carcinoma is the predominant cancer among malignancies in women throughout the world, and human papillomavirus (HPV) 16 is the most common agent linked to human cervical carcinoma. The present study was performed to investigate the mechanisms of immune escape in HPV-induced cervical cancer cells. The presence of HPV oncoproteins E6 and E7 in the extracellular fluids of HPV-containing cervical cancer cell lines SiHa and CaSki was demonstrated by ELISA. The effect of HPV 16 oncoproteins E6 and E7 on the production of IFN-γ by IL-18 was assessed. E6 and E7 proteins reduced IL-18-induced IFN-γ production in both primary PBMCs and the NK0 cell line. FACS analysis revealed that the viral oncoproteins reduced the binding of IL-18 to its cellular surface receptors on NK0 cells, whereas there was no effect of oncoproteins on IL-1 binding to its surface IL-1 receptors on D10S, a subclone of the murine Th cell D10.G4.1. In vitro pull-down assays also revealed that the viral oncoproteins and IL-18 bound to IL-18R α-chain competitively. These results suggest that the extracellular HPV 16 E6 and E7 proteins may inhibit IL-18-induced IFN-γ production locally in HPV lesions through inhibition of IL-18 binding to its α-chain receptor. Down-modulation of IL-18-induced immune responses by HPV oncoproteins may contribute to viral pathogenesis or carcinogenesis.
ISSN
0022-1767
Publisher
Amer Assoc Immunologists
DOI
http://dx.doi.org/10.4049/jimmunol.167.1.497
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.