Determination of rat leptin activity in vitro using a novel luciferase reporter assay

Cited 0 time in scopus
Metadata Downloads
Title
Determination of rat leptin activity in vitro using a novel luciferase reporter assay
Author(s)
Sung Kyu Ju; Jung Hyun Park; Shin Young Na; Kwan Hee You; Kil Lyong Kim; Myung Kyu Lee
Bibliographic Citation
Molecules and Cells, vol. 12, no. 1, pp. 131-136
Publication Year
2001
Abstract
Leptin is small cytokine-like protein that is involved in appetite and body weight regulation. Due to increased interest in using leptin as an anti-obesity reagent, recombinant forms of leptin have been produced for several species, including humans, mice, rats, pigs, dogs, sheep etc. The biological activities of such recombinant proteins were determined using various in vitro or in vivo systems; however so far, no specific assay system for rat leptin is available. Since rats are representative animal models in obesity research, the establishment of a biological assay system for determining rat leptin activity has been eagerly awaited. This study describes the generation of such a system using chinese hamster ovary (CHO)-cells that were transfected with the long form of the rat leptin receptor isoform, OB-Rb, whereby a signal transduces and activators of transcription-sensitive luciferase reporter system is further employed to quantify the leptin-mediated signals. This system is the first rat-specific leptin bioassay system that has been reported. It is expected that this assay will be used to further quantify and determine leptin activity from various biological fluids and sources.
Keyword
leptinluciferaseOB-Rbratreceptor
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.