Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences

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dc.contributor.authorMoon Sun Hahm-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T08:58:22Z-
dc.date.available2017-04-19T08:58:22Z-
dc.date.issued2001-
dc.identifier.issn1226-8372-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5576-
dc.description.abstractA recombinant human growth hormone (hGH) was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase and invertase were used to direct the secretion of hGH into the extracellular medium. Among three leader sequences tested, the inulinase leader sequence was found to be the most efficient in the secretory expression of hGH. In contrast, no hGH was detected in the extracellular medium with the invertase leader sequence. After 48 h shake-flask culture, the yields of hGH secreted into the medium by the invertase, MFα1, inulinase and invertase leader sequences were approximately 0, 0.3 and 0.9 mg/L, respectively. The secretion efficiencies were also found to be 0, 3.8 and 13% for the invertase, MFα1 and inulinase leader sequences, respectively.-
dc.publisherSpringer-
dc.titleSecretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences-
dc.title.alternativeSecretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences-
dc.typeArticle-
dc.citation.titleBiotechnology and Bioprocess Engineering-
dc.citation.number4-
dc.citation.endPage309-
dc.citation.startPage306-
dc.citation.volume6-
dc.contributor.affiliatedAuthorMoon Sun Hahm-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.alternativeName함문선-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationBiotechnology and Bioprocess Engineering, vol. 6, no. 4, pp. 306-309-
dc.identifier.doi10.1007/BF02931995-
dc.subject.keywordhuman growth hormone-
dc.subject.keywordsecretion-
dc.subject.keywordleader sequence-
dc.subject.keywordSaccharomyces cerevisiae-
dc.subject.localhuman growth hormone-
dc.subject.localsecretion-
dc.subject.localSecretion-
dc.subject.localleader sequence-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localsaccharomyces cerevisiae-
dc.description.journalClassY-
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