DC Field | Value | Language |
---|---|---|
dc.contributor.author | Moon Sun Hahm | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.date.accessioned | 2017-04-19T08:58:22Z | - |
dc.date.available | 2017-04-19T08:58:22Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5576 | - |
dc.description.abstract | A recombinant human growth hormone (hGH) was expressed as a secretory product in the yeast Saccharomyces cerevisiae. Three different leader sequences derived from the mating factor α1 (MFα1), inulinase and invertase were used to direct the secretion of hGH into the extracellular medium. Among three leader sequences tested, the inulinase leader sequence was found to be the most efficient in the secretory expression of hGH. In contrast, no hGH was detected in the extracellular medium with the invertase leader sequence. After 48 h shake-flask culture, the yields of hGH secreted into the medium by the invertase, MFα1, inulinase and invertase leader sequences were approximately 0, 0.3 and 0.9 mg/L, respectively. The secretion efficiencies were also found to be 0, 3.8 and 13% for the invertase, MFα1 and inulinase leader sequences, respectively. | - |
dc.publisher | Springer | - |
dc.title | Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences | - |
dc.title.alternative | Secretory expression of human growth hormone in Saccharomyces cerevisiae using three different leader sequences | - |
dc.type | Article | - |
dc.citation.title | Biotechnology and Bioprocess Engineering | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 309 | - |
dc.citation.startPage | 306 | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Moon Sun Hahm | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.alternativeName | 함문선 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.identifier.bibliographicCitation | Biotechnology and Bioprocess Engineering, vol. 6, no. 4, pp. 306-309 | - |
dc.identifier.doi | 10.1007/BF02931995 | - |
dc.subject.keyword | human growth hormone | - |
dc.subject.keyword | secretion | - |
dc.subject.keyword | leader sequence | - |
dc.subject.keyword | Saccharomyces cerevisiae | - |
dc.subject.local | human growth hormone | - |
dc.subject.local | secretion | - |
dc.subject.local | Secretion | - |
dc.subject.local | leader sequence | - |
dc.subject.local | Saccharomyces cerevisiae | - |
dc.subject.local | saccharomyces cerevisiae | - |
dc.description.journalClass | Y | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.