Production of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber = 오이의 배발생 현탁 배양세포로부터 제초제 저항성 형질전환 식물체 생산

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dc.contributor.authorJe Wook Woo-
dc.contributor.authorWon Joong Chung-
dc.contributor.authorKwan Sam Choi-
dc.contributor.authorHyo Guen Park-
dc.contributor.authorNam Kwon Baek-
dc.contributor.authorJang Ryol Liu-
dc.date.accessioned2017-04-19T08:58:23Z-
dc.date.available2017-04-19T08:58:23Z-
dc.date.issued2001-
dc.identifier.issn10155880-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5582-
dc.description.abstractTo develop herbicide-resistant cucumber plants (Cucumis sativus L. cv Green Angle) embryogenic suspension cultures were co-cultured with Agrobacterium tumefaciens strain LBA4404 carrying a disarmed binary vector pGA-bar. The T-DNA region of this binary vector contains the nopalin synthase/neomycin phosphotransferase Ⅱ (npt Ⅱ) chimeric gene for kanamycin resistance and the cauliflower 35S/phosphinothricin acetyltransferase (bar) chimeric gene for phosphinothricin (PPT) resistance, After co-cultivation for 48 h, embryogenic calli were placed on maturation media containing 20 mg/L PPT. Approximately 200 putatively transgenic plantlets were obtained in hormone free media containing 40 mg/L PPT. Northern blot hybridization analysis confirmed the expression of the bar gene that was integrated into the genome of five transgenic plants. Transgenic cucumber plants were grown to maturity. Mature plants in soil showed tolerance to the commercial herbicide (Basta) of PPT at the manufacturer's suggested level (3 mL/L).-
dc.publisherSouth Korea-
dc.titleProduction of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber = 오이의 배발생 현탁 배양세포로부터 제초제 저항성 형질전환 식물체 생산-
dc.title.alternativeProduction of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber-
dc.typeArticle-
dc.citation.titleKorean Journal of Plant Biotechnology (Tissue Culture)-
dc.citation.number1-
dc.citation.endPage58-
dc.citation.startPage53-
dc.citation.volume28-
dc.contributor.affiliatedAuthorWon Joong Chung-
dc.contributor.alternativeName우제욱-
dc.contributor.alternativeName정원중-
dc.contributor.alternativeName최관삼-
dc.contributor.alternativeName박효근-
dc.contributor.alternativeName백남권-
dc.contributor.alternativeName유장렬-
dc.identifier.bibliographicCitationKorean Journal of Plant Biotechnology (Tissue Culture), vol. 28, no. 1, pp. 53-58-
dc.subject.keywordagrobacterium tumefaciens-
dc.subject.keywordcucumis sativus L.-
dc.subject.keywordherbicide-resistance-
dc.subject.keywordsomatic embryogenesis-
dc.subject.keywordtransformation-
dc.subject.localagrobacterium tumefaciens-
dc.subject.localAgrobacterium tumefaciens-
dc.subject.localcucumis sativus L.-
dc.subject.localCucumis sativus L.-
dc.subject.localherbicide-resistance-
dc.subject.localHerbicide resistance-
dc.subject.localsomatic embryogenesis-
dc.subject.localSomatic embryogenesis-
dc.subject.localtransformation-
dc.subject.localTransformation-
dc.description.journalClassN-
Appears in Collections:
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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