DC Field | Value | Language |
---|---|---|
dc.contributor.author | Je Wook Woo | - |
dc.contributor.author | Won Joong Chung | - |
dc.contributor.author | Kwan Sam Choi | - |
dc.contributor.author | Hyo Guen Park | - |
dc.contributor.author | Nam Kwon Baek | - |
dc.contributor.author | Jang Ryol Liu | - |
dc.date.accessioned | 2017-04-19T08:58:23Z | - |
dc.date.available | 2017-04-19T08:58:23Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 1015-5880 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5582 | - |
dc.description.abstract | To develop herbicide-resistant cucumber plants (Cucumis sativus L. cv Green Angle) embryogenic suspension cultures were co-cultured with Agrobacterium tumefaciens strain LBA4404 carrying a disarmed binary vector pGA-bar. The T-DNA region of this binary vector contains the nopalin synthase/neomycin phosphotransferase Ⅱ (npt Ⅱ) chimeric gene for kanamycin resistance and the cauliflower 35S/phosphinothricin acetyltransferase (bar) chimeric gene for phosphinothricin (PPT) resistance, After co-cultivation for 48 h, embryogenic calli were placed on maturation media containing 20 mg/L PPT. Approximately 200 putatively transgenic plantlets were obtained in hormone free media containing 40 mg/L PPT. Northern blot hybridization analysis confirmed the expression of the bar gene that was integrated into the genome of five transgenic plants. Transgenic cucumber plants were grown to maturity. Mature plants in soil showed tolerance to the commercial herbicide (Basta) of PPT at the manufacturer's suggested level (3 mL/L). | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Production of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber = 오이의 배발생 현탁 배양세포로부터 제초제 저항성 형질전환 식물체 생산 | - |
dc.title.alternative | Production of herbicide-resistant transgenic plants from embryogenic suspension cultures of cucumber | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Plant Biotechnology (Tissue Culture) | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 58 | - |
dc.citation.startPage | 53 | - |
dc.citation.volume | 28 | - |
dc.contributor.affiliatedAuthor | Je Wook Woo | - |
dc.contributor.affiliatedAuthor | Won Joong Chung | - |
dc.contributor.affiliatedAuthor | Jang Ryol Liu | - |
dc.contributor.alternativeName | 우제욱 | - |
dc.contributor.alternativeName | 정원중 | - |
dc.contributor.alternativeName | 최관삼 | - |
dc.contributor.alternativeName | 박효근 | - |
dc.contributor.alternativeName | 백남권 | - |
dc.contributor.alternativeName | 유장렬 | - |
dc.identifier.bibliographicCitation | Korean Journal of Plant Biotechnology (Tissue Culture), vol. 28, no. 1, pp. 53-58 | - |
dc.subject.keyword | agrobacterium tumefaciens | - |
dc.subject.keyword | cucumis sativus L. | - |
dc.subject.keyword | herbicide-resistance | - |
dc.subject.keyword | somatic embryogenesis | - |
dc.subject.keyword | transformation | - |
dc.subject.local | Agrobacterium tumefaciens | - |
dc.subject.local | agrobacterium tumefaciens | - |
dc.subject.local | Cucumis sativus | - |
dc.subject.local | Cucumis sativus L. | - |
dc.subject.local | cucumis sativus | - |
dc.subject.local | cucumis sativus L. | - |
dc.subject.local | Herbicide resistance | - |
dc.subject.local | herbicide resistance | - |
dc.subject.local | herbicide-resistance | - |
dc.subject.local | Somatic embryogenesis | - |
dc.subject.local | somatic embryogenesis | - |
dc.subject.local | somatic embryogenisis | - |
dc.subject.local | Transformation | - |
dc.subject.local | transformation | - |
dc.description.journalClass | N | - |
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