Enantioselective hydrolysis of racemic naproxen methyl ester by two-step acetone-treated Candida rugosa lipase

Cited 24 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorEun Gyo Lee-
dc.contributor.authorHye Soon Won-
dc.contributor.authorBong Hyun Chung-
dc.date.accessioned2017-04-19T08:58:25Z-
dc.date.available2017-04-19T08:58:25Z-
dc.date.issued2001-
dc.identifier.issn00329592-
dc.identifier.uri10.1016/S0032-9592(01)00213-8ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5593-
dc.description.abstractAn optically pure (S)-naproxen was produced by two-step acetone-treated Candida rugosa lipase (CRL) through enantioselective hydrolysis of racemic naproxen methyl ester. The two-step acetone-treated CRL was much more enantioselective than the crude CRL towards the hydrolysis of (R,S)-naproxen methyl ester, yielding an enantiomeric excess (eep) of > 98% and an enantiomeric ratio (E) of > 100. In terms of hydrolysis reaction rate and enantioselectivity, the optimal reaction conditions were found to be 37 °C and pH 6.0. The scaled-up production of (S)-naproxen was performed in a batch reactor containing 200 ml of substrate solution (50 mM MES buffer containing 200 mM (R,S)-naproxen methyl ester). After 156 h of reaction, 38.4% of the initial (R,S)-naproxen methyl ester was hydrolyzed, yielding an enantiomeric excess of 98% and an enantiomeric ratio of > 100. Finally, (S)-naproxen was recovered from the reaction mixture with an optical purity of 98% and a recovery yield of 95%.-
dc.publisherElsevier-
dc.titleEnantioselective hydrolysis of racemic naproxen methyl ester by two-step acetone-treated Candida rugosa lipase-
dc.title.alternativeEnantioselective hydrolysis of racemic naproxen methyl ester by two-step acetone-treated Candida rugosa lipase-
dc.typeArticle-
dc.citation.titleProcess Biochemistry-
dc.citation.number3-
dc.citation.endPage298-
dc.citation.startPage293-
dc.citation.volume37-
dc.contributor.affiliatedAuthorEun Gyo Lee-
dc.contributor.alternativeName이은교-
dc.contributor.alternativeName원혜순-
dc.contributor.alternativeName정봉현-
dc.identifier.bibliographicCitationProcess Biochemistry, vol. 37, no. 3, pp. 293-298-
dc.identifier.doi10.1016/S0032-9592(01)00213-8-
dc.subject.keywordcandida rugosa lipase (CRL)-
dc.subject.keywordenantioselective hydrolysis-
dc.subject.keyword(S)-Naproxen-
dc.subject.keyword(R,S)-Naproxen methyl ester-
dc.subject.keywordtwo-step acetone treatment-
dc.subject.localcandida rugosa lipase (CRL)-
dc.subject.localCandida rugosa lipase-
dc.subject.localenantioselective hydrolysis-
dc.subject.localEnantioselective hydrolysis-
dc.subject.local(S)-Naproxen-
dc.subject.local(R,S)-Naproxen methyl ester-
dc.subject.localtwo-step acetone treatment-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.