Cyclophilin A binds to peroxiredoxins and activates its peroxidase activity

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Cyclophilin A binds to peroxiredoxins and activates its peroxidase activity
Sang Pil Lee; Young Sun Hwang; Yong Jun Kim; Ki Sun Kwon; Hyung Jung Kim; Kang Hwa Kim; Ho Zoon Chae
Bibliographic Citation
Journal of Biological Chemistry, vol. 276, no. 32, pp. 29826-29832
Publication Year
Six distinct peroxiredoxin (Prx) proteins (Prx I-VI) from distinct genes have been identified in mammalian tissues. Prxs are members of a group of peroxidases that have conserved reactive cysteine residue(s) in the active site(s). An immediate physiological electron donor for the peroxidase catalysis for five Prx proteins (Prx I-V) has been identified as thioredoxin (Trx), but that for Prx VI (1-Cys Prx) is still unclear. To identify an immediate electron donor and a binding protein for Prx VI, we performed a Prx VI protein overlay assay. A 20-kDa binding protein was identified by the Prx VI protein overlay assay with flow-through fractions from a High-Q column with rat lung crude extracts. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and MS-Fit, we identified the 20-kDa Prx VI-binding protein as a cyclophilin A (CyP-A). The binding of recombinant human CyP-A (hCyP-A) to Prx VI was confirmed by using the hCyP-A protein over-lay assay and Western immunoblot analysis with hCyP-A-specific antibodies. hCyP-A enhanced the antioxidant activity of Prx VI, as well as the other known mammalian Prx isotypes. hCyP-A supported antioxidant activity of Prx II and Prx VI both against thiol (dithiothreitol)-containing metal-catalyzed oxidation (MCO) systems and ascorbate-containing MCO systems. Prx II was reduced by hCyP-A without help from any other reductant, and the reduction was cyclosporin A-independent. These results strongly suggest that CyP-A not only binds to Prx proteins but also supports its peroxidase activity as an immediate electron donor. In addition, Cys115 and Cys161 of hCyP-A were found to be involved in the activation and the reduction of Prx.
Amer Soc Biochemistry Molecular Biology Inc
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Aging Convergence Research Center > 1. Journal Articles
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