Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha

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Title
Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha
Author(s)
M D Kim; Kang Hyun Ah; Sang Ki Rhee; J H Seo
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 27, no. 1, pp. 58-61
Publication Year
2001
Abstract
A series of batch, fed-batch, and continuous cultures was carried out to analyze the effects of methanol on the fermentation characteristics of recombinant Hansenula polymorpha for the production of hirudin, an anticoagulant. Hirudin expression efficiencies were greatly influenced by the methanol concentrations in continuous and fed-batch culture modes. At a steady state of continuous culture, an optimum methanol concentration of 1.7 g I-1 was determined at a dilution rate of 0.18 h-1 with 1.8 mg I-1 h-1 hirudin productivity.
Keyword
Hansenula polymorphaHirudinMethanolMethanol oxidase promoter
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1038/sj.jim.7000163
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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