Characterization of a cell line HFH-T2, producing viral particles, from primary human fetal hepatocytes infected with hepatitis B virus

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Title
Characterization of a cell line HFH-T2, producing viral particles, from primary human fetal hepatocytes infected with hepatitis B virus
Author(s)
Jae Kyoung Shim; Dong Wook Kim; Tae Ho Chung; June Ki Kim; Jeong Ill Suh; Chun Park; Young Choon Lee; Tai Wha Chung; Eun Young Song; Chul Ho Kim
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 11, no. 2, pp. 186-192
Publication Year
2001
Abstract
A primary culture of human fetal hepatocytes was obtained though a therapeutic abortion process at 26 weeks of gestation period. More than 108 cells were seeded on a plastic plate. These hepatocytes were infected with hepatitis B virus (HBV). The HBV was purified from serum of one chronic HBV carrier. Transformed hepatocytes were subcultured in a 10% FBS-supplemented medium. The morphology of the transformed cell was epithelial-like. The cells from the first pass showed signs of early proliferation and had a latent period of more than 3 months after 6-7 passages. After the rest period, the transformed cell proliferated actively and they were subcultured every three days. Transformed hepatocytes were characterized by detection of the HBV transcript by RT-PCR. The secretion of virions from transformed cells was investigated by PCR with the cell medium. Two types of virions secreted into the culture medium were examined by using the transmission electron microscope. Another approach to study the secretion of virions in to culture medium was carried out with HBV antibody. HBsAg was detected in the culture medium of transformed cells using ELISA and Western blot analyses. These data suggested that the human fetal hepatocyte cell line has been established by infection of HBV, in which this cell line secreted viral particles into the culture medium.
Keyword
dan particleenzyme-linked immunosorbent assayfetal hepatocyteHBsAgHBsAg particlehepatitis B virushepatocellular carcinomanatural infection
ISSN
1017-7825
Publisher
South Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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