Polychlorinated biphenyls activate caspase-3-like death protease in Vitro but not in Vivo

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Title
Polychlorinated biphenyls activate caspase-3-like death protease in Vitro but not in Vivo
Author(s)
Sang-Han Lee; Eun Soo Youk; Young Jin Jeon; Sang Bae Han; Hyoung-Chin Kim; Hwan Mook Kim
Bibliographic Citation
Biological & Pharmaceutical Bulletin, vol. 24, no. 12, pp. 1380-1383
Publication Year
2001
Abstract
We prove here that serum albumin inhibits apoptosis induced by polychlorinated biphenyls (PCBs), confirming that serum albumin binds to PCB, and that the albumin-PCB complexes inhibit apoptosis in HL-60 cells. We found that PCB (50 μM) increased the activity of caspase-3-like protease when HL-60 cells, as well as splenocytes, were cultured in "serum-free medium." Benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk) inhibited apoptosis in cells cultured in the serum-free medium containing 50 μM PCB. To elucidate whether or not PCBs induce apoptosis in vivo, we examined apoptosis of splenocytes by administering PCB to ICR mice (100, 500, 1000 mg·kg-1·d-1) for 5 d and characterizing splenocytes. Interestingly, splenocytes treated with PCB did not show any changes characteristic of apoptosis. These results demonstrate that PCB activates the caspase-3-like death protease in vitro in serum-free medium, but does not induce apoptosis of splenocytes in vivo, suggesting that blood serum may mask the apoptosis induced by PCB.
Keyword
Caspase-3-like proteasePolychlorinated biphenylSerum albuminSplenocyte
ISSN
0918-6158
Publisher
Pharmaceutical Soc Japan
DOI
http://dx.doi.org/10.1248/bpb.24.1380
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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