Effect of DNA conformation on genomic integration of transgenes and its implications on integration mechanism = 외래유전자의 게놈내 삽입에 있어서 DNA 형태가 미치는 영향

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dc.contributor.authorYong Kook Kang-
dc.contributor.authorJung Sun Park-
dc.contributor.authorChul Sang Lee-
dc.contributor.authorYong Mahn Han-
dc.contributor.authorKyung Kwang Lee-
dc.date.accessioned2017-04-19T08:58:34Z-
dc.date.available2017-04-19T08:58:34Z-
dc.date.issued2001-
dc.identifier.issnI0000006-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5656-
dc.description.abstractWe recently demonstrated that both linear- and supercoil-form B1/B2 SINE (short interspersed elements) sequenced could increase an intergration frequency of a reporter gene in preimplatation mouse embroys. In those reports, when either a control or SINE-flanked DNA was separately applied to microinjection, the proportions of β-gal positive were 16% and 63%, respectively, in liner-formDNA, and 6% and 25%, respectively, in circular-form DNA. Here, we examined the contribution of a circular-form DNA moiety to integration frequency by using a mixed-form (linear and circular-form) DNA in microinjection. When examined in the blastocyst stage, the proportion of β-gal-positive embroyos was 17.3% and 46.6% in control and SINE-flanked DNA, respectively. These results suggest that there is little contribution of circular-form DNA moiety to the resultant intergration frequency, and that the majority of the integration events are mediated through a linear confornation of vector DNA. In addition, some clues on integration process could be obtained from the analysis of microinjection results.-
dc.publisherSouth Korea-
dc.titleEffect of DNA conformation on genomic integration of transgenes and its implications on integration mechanism = 외래유전자의 게놈내 삽입에 있어서 DNA 형태가 미치는 영향-
dc.title.alternativeEffect of DNA conformation on genomic integration of transgenes and its implications on integration mechanism-
dc.typeArticle-
dc.citation.titleReproductive & Developmental Biology-
dc.citation.number3-
dc.citation.endPage242-
dc.citation.startPage237-
dc.citation.volume25-
dc.contributor.affiliatedAuthorYong Kook Kang-
dc.contributor.affiliatedAuthorJung Sun Park-
dc.contributor.alternativeName강용국-
dc.contributor.alternativeName박정선-
dc.contributor.alternativeName이철상-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeName이경광-
dc.identifier.bibliographicCitationReproductive & Developmental Biology, vol. 25, no. 3, pp. 237-242-
dc.description.journalClassN-
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Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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