Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography

Cited 44 time in scopus
Metadata Downloads
Title
Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography
Author(s)
Jong Seong Kang; Pham Tuan Linh; Xing Fu Cai; Hang Sub Kim; Jung Joon Lee; Young Ho Kim
Bibliographic Citation
Archives of Pharmacal Research, vol. 24, no. 5, pp. 407-411
Publication Year
2001
Abstract
Reversed-phase high performance liquid Chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected in Korea. The stationary phase used was Zorbax 300 SB C18 and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and E in stems were higher than chose in roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.
Keyword
acanthopanax specieseleutheroside beleutheroside egradient elutionHPLC
ISSN
0253-6269
Publisher
Pharmaceutical Soc Korea
DOI
http://dx.doi.org/10.1007/BF02975184
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.