Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.

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Title
Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.
Author(s)
Young Min Ha; Dong Gun Lee; Jung Hoon Yoon; Yong Ha Park; Young Jae Kim
Bibliographic Citation
Biotechnology Letters, vol. 23, no. 17, pp. 1435-1438
Publication Year
2001
Abstract
Bacillus sp. KYJ 963,a local isolate, produced an extracellular amylase with Mr = 59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.
Keyword
anchovy-jeotbacillus sp. KYJ 963extracellular β-amylasestarch adsorption
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1011682223796
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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