Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase

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dc.contributor.authorJin Koo Kim-
dc.contributor.authorKwang Dong Kim-
dc.contributor.authorJong-Seok Lim-
dc.contributor.authorHee Gu Lee-
dc.contributor.authorSang Jae Kim-
dc.contributor.authorSang Hyun Cho-
dc.contributor.authorWon Hwa Jeong-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorTai Wha Chung-
dc.contributor.authorSang Gi Paik-
dc.contributor.authorYong Kyung Choe-
dc.date.accessioned2017-04-19T08:58:39Z-
dc.date.available2017-04-19T08:58:39Z-
dc.date.issued2001-
dc.identifier.issn1225-8687-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5685-
dc.description.abstractThe Mycobacterium bovis BCG panB gene, encoding ketopantoate hydroxymethyltransferase (KPHMT), was cloned from a λgt11 genomic library and sequenced. The DNA sequence encodes a protein that contains 281 amino acid residues (Mr 29,337) with a high similarity to the KPHMTs. Subcloning of a 846 bp open reading frame (ORF), but not a 735 bp ORF, into the vector pUC19 led to complementation of the panB mutant of Escherichia coli. The BCG panB gene was overexpressed in E. coli and the KPHMT purified to homogeneity. The recombinant protein was further confirmed by an enzymatic assay.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleCloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase-
dc.title.alternativeCloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxylmethyltransferase-
dc.typeArticle-
dc.citation.titleBMB Reports-
dc.citation.number4-
dc.citation.endPage346-
dc.citation.startPage342-
dc.citation.volume34-
dc.contributor.affiliatedAuthorJin Koo Kim-
dc.contributor.affiliatedAuthorKwang Dong Kim-
dc.contributor.affiliatedAuthorJong-Seok Lim-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.affiliatedAuthorYong Kyung Choe-
dc.contributor.alternativeName김진구-
dc.contributor.alternativeName김광동-
dc.contributor.alternativeName임종석-
dc.contributor.alternativeName이희구-
dc.contributor.alternativeName김상재-
dc.contributor.alternativeName조상현-
dc.contributor.alternativeName정원화-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName정태화-
dc.contributor.alternativeName백상기-
dc.contributor.alternativeName최용경-
dc.identifier.bibliographicCitationBMB Reports, vol. 34, no. 4, pp. 342-346-
dc.subject.keywordBCG-
dc.subject.keywordKetopantoate hydroxymethyltransferase (KPHMT)-
dc.subject.keywordpanB-
dc.subject.keywordPantothenic acid-
dc.subject.localBCG-
dc.subject.localKetopantoate hydroxymethyltransferase (KPHMT)-
dc.subject.localpanB-
dc.subject.localPantothenic acid-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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