Levan production by use of the recombinant levansucrase immobilized on titanium-activated magnetite

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Title
Levan production by use of the recombinant levansucrase immobilized on titanium-activated magnetite
Author(s)
Ki Hyo Jang; Ki Bang Song; Buem Seek Park; Chul Ho Kim; Bong Hyun Chung; Ryo Won Choue; Kwang Suck Lee; Chan Lee; Uck Han Chun; Sang Ki Rhee
Bibliographic Citation
Process Biochemistry, vol. 37, no. 4, pp. 339-343
Publication Year
2001
Abstract
Levansucrase from Zymomonas mobilis expressed in Escherichia coli was immobilized onto the surface of magnetite. Optimum conditions for the immobilization were, pH 4.0; immobilization reaction time of 30 min and 8 U of enzyme per gram of matrix. Immobilization yield was 75% under optimized conditions. The maximum production yield of levan by the immobilized levansucrase was about 42% on a fructose released basis with sucrose as substrate at 100 g/l. Thermal stability of the levansucrase increased by immobilization procedure. Furthermore, immobilized enzyme showed the less polymerizing activity in levan formation, producing the low-molecular weight (MW) levan. The molecular weight of levan can be controlled by using the immobilized levansucrase in high yield. Immobilized levansucrase retained 61% of the original activity after five repeated uses.
Keyword
ImmobilizationLevanLevansucraseTitanium-activated magnetiteZymomonas mobilis
ISSN
0032-9592
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0032-9592(01)00215-1
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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