Rapid isolation of genomic DNA from normal and apoptotic cells using magnetic silica resins

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorJee Sun Park-
dc.contributor.authorJung Hyun Park-
dc.contributor.authorShin Young Na-
dc.contributor.authorSoo Young Choe-
dc.contributor.authorSang Nam Choi-
dc.contributor.authorKwan Hee You-
dc.date.accessioned2017-04-19T08:58:41Z-
dc.date.available2017-04-19T08:58:41Z-
dc.date.issued2001-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5695-
dc.description.abstractThe isolation of genomic DNA from mammalian cells is usually performed by cell lysis followed by protein digestion, extraction, and finally, ethanol precipitation of the chromosomal DNA. However, in the case of large sample numbers or when fast processing is required or when only small amounts of starting materials are available, such conventional methods are not efficient and are cumbersome to be applied. Some alternative methods have been described as well as having commercial DNA isolation kits to be available, nevertheless, there is room left for much improvement. In the present study, a novel method is introduced, where it simplifies conventional protocols by omitting some time-consuming steps such as protease incubation or DNA precipitation and its resuspension. Using paramagnetic silica resins, the genomic DNA was purified over a magnetic field, and the bound DNA was eluted with a low-salt buffer. The fidelity and effectiveness of this novel method was determined by using normal and apoptotic cells as a starting material and then compared to other protocols. The high speed and convenience along with its high efficiency in detecting apoptotic chromosomal DNA will prove this method to be an improved alternative in the isolation of genomic DNA from mammalian cells.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleRapid isolation of genomic DNA from normal and apoptotic cells using magnetic silica resins-
dc.title.alternativeRapid isolation of genomic DNA from normal and apoptotic cells using magnetic silica resins-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number5-
dc.citation.endPage894-
dc.citation.startPage890-
dc.citation.volume11-
dc.contributor.affiliatedAuthorJung Hyun Park-
dc.contributor.alternativeName박지선-
dc.contributor.alternativeName박정현-
dc.contributor.alternativeName나신영-
dc.contributor.alternativeName최수영-
dc.contributor.alternativeName최상남-
dc.contributor.alternativeName유관희-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 11, no. 5, pp. 890-894-
dc.subject.keywordapoptosis-
dc.subject.keyworddexamethasone-
dc.subject.keywordgenomic DNA-
dc.subject.keywordjurkat cells-
dc.subject.keywordmagnetic silica-
dc.subject.localApoptosis-
dc.subject.localapoptosis-
dc.subject.localdexamethasone-
dc.subject.localDexamethasone-
dc.subject.localGenomic DNA-
dc.subject.localgenomic DNA-
dc.subject.localJurkat cell-
dc.subject.localJurkat cells-
dc.subject.localjurkat cells-
dc.subject.localmagnetic silica-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.