DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jong Pil Park | - |
dc.contributor.author | Tae Kwang Oh | - |
dc.contributor.author | Jong Won Yun | - |
dc.date.accessioned | 2017-04-19T08:58:41Z | - |
dc.date.available | 2017-04-19T08:58:41Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0032-9592 | - |
dc.identifier.uri | 10.1016/S0032-9592(01)00237-0 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/5697 | - |
dc.description.abstract | A novel transfructosylating enzyme, which produces fructo-oligosaccharides from sucrose from Bacillus macerans EG-6 were purified 63.5-fold by ammonium sulphate precipitation (20-60%), CM-Sepharose CL 6B and fast protein liquid chromatographies on Resource Q, Phenyl-Superose HR 5/5 and Mono S (Pharmacia, Uppsala, Sweden). The minimum molecular mass of the purified enzyme was 66 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was stable at the pH range of 5.0-7.0 and had an optimum pH at 5.0. The optimum temperature for enzyme activity was at 50°C. The oligosaccharide compositions in reaction products were significantly different on using the enzyme obtained from each purification step. For example, crude enzyme unusually produced selectively GF5- and GF6-fructo-oligosaccharide whereas purified enzyme produced mainly 1-kestose (GF2) and nystose (GF3) as in the case of other transfructosylating enzymes. | - |
dc.publisher | Elsevier | - |
dc.title | Purification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6 | - |
dc.title.alternative | Purification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6 | - |
dc.type | Article | - |
dc.citation.title | Process Biochemistry | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 476 | - |
dc.citation.startPage | 471 | - |
dc.citation.volume | 37 | - |
dc.contributor.affiliatedAuthor | Tae Kwang Oh | - |
dc.contributor.alternativeName | 박종필 | - |
dc.contributor.alternativeName | 오태광 | - |
dc.contributor.alternativeName | 윤종원 | - |
dc.identifier.bibliographicCitation | Process Biochemistry, vol. 37, no. 5, pp. 471-476 | - |
dc.identifier.doi | 10.1016/S0032-9592(01)00237-0 | - |
dc.subject.keyword | bacillus macerans EG-6 | - |
dc.subject.keyword | fructo-oligosaccharide | - |
dc.subject.keyword | purification | - |
dc.subject.keyword | transfructosylating enzyme | - |
dc.subject.local | bacillus macerans EG-6 | - |
dc.subject.local | fructooligosaccharides (FOS) | - |
dc.subject.local | fructo-oligosaccharide | - |
dc.subject.local | Fructooligosaccharides | - |
dc.subject.local | Purification | - |
dc.subject.local | purification | - |
dc.subject.local | Purifcation | - |
dc.subject.local | transfructosylating enzyme | - |
dc.description.journalClass | Y | - |
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