Purification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6

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dc.contributor.authorJong Pil Park-
dc.contributor.authorTae Kwang Oh-
dc.contributor.authorJong Won Yun-
dc.date.accessioned2017-04-19T08:58:41Z-
dc.date.available2017-04-19T08:58:41Z-
dc.date.issued2001-
dc.identifier.issn0032-9592-
dc.identifier.uri10.1016/S0032-9592(01)00237-0ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/5697-
dc.description.abstractA novel transfructosylating enzyme, which produces fructo-oligosaccharides from sucrose from Bacillus macerans EG-6 were purified 63.5-fold by ammonium sulphate precipitation (20-60%), CM-Sepharose CL 6B and fast protein liquid chromatographies on Resource Q, Phenyl-Superose HR 5/5 and Mono S (Pharmacia, Uppsala, Sweden). The minimum molecular mass of the purified enzyme was 66 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was stable at the pH range of 5.0-7.0 and had an optimum pH at 5.0. The optimum temperature for enzyme activity was at 50°C. The oligosaccharide compositions in reaction products were significantly different on using the enzyme obtained from each purification step. For example, crude enzyme unusually produced selectively GF5- and GF6-fructo-oligosaccharide whereas purified enzyme produced mainly 1-kestose (GF2) and nystose (GF3) as in the case of other transfructosylating enzymes.-
dc.publisherElsevier-
dc.titlePurification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6-
dc.title.alternativePurification and characterization of a novel transfructosylating enzyme from Bacillus macerans EG-6-
dc.typeArticle-
dc.citation.titleProcess Biochemistry-
dc.citation.number5-
dc.citation.endPage476-
dc.citation.startPage471-
dc.citation.volume37-
dc.contributor.affiliatedAuthorTae Kwang Oh-
dc.contributor.alternativeName박종필-
dc.contributor.alternativeName오태광-
dc.contributor.alternativeName윤종원-
dc.identifier.bibliographicCitationProcess Biochemistry, vol. 37, no. 5, pp. 471-476-
dc.identifier.doi10.1016/S0032-9592(01)00237-0-
dc.subject.keywordbacillus macerans EG-6-
dc.subject.keywordfructo-oligosaccharide-
dc.subject.keywordpurification-
dc.subject.keywordtransfructosylating enzyme-
dc.subject.localbacillus macerans EG-6-
dc.subject.localfructooligosaccharides (FOS)-
dc.subject.localfructo-oligosaccharide-
dc.subject.localFructooligosaccharides-
dc.subject.localPurification-
dc.subject.localpurification-
dc.subject.localPurifcation-
dc.subject.localtransfructosylating enzyme-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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